S22 Micromanipulation of mammalian embryos

Immunocytochemistry in Early Mammalian Embryos

Electroporation into Cultured Mammalian Embryos

Over the last century, mammalian embryos have been used extensively as a common animal model to investigate fundamental questions in the field of developmental biology. More recently, the establishment of transgenic and gene-targeting systems in laboratory mice has enabled researchers to unveil the genetic mechanisms underlying complex developmental processes (Mak, 2007). However, our understan...

Adaptive harmonic generation microscopy of mammalian embryos.

Adaptive optics is implemented in a harmonic generation microscope using a wavefront sensorless correction scheme. Both the second- and third-harmonic intensity signals are used as the optimization metric. Aberration correction is performed to compensate both system- and specimen-induced aberrations by using an efficient optimization routine based upon Zernike polynomial modes. Images of live m...

Cryopreservation of Mammalian Embryos by Vitrification

protectant for spermatozoa41) led to research on cryopreservation of mammalian embryos. In the past quarter of a century since the first successful cryopreservation of mouse embryos58,60), basic and applied research has resulted in the cryopreservation of embryos of many mammalian species. Improvements have been made in nearly all steps of the cryopreservation process, resulting in considerable...

Ongoing Pregnancies following Cosmetic Micromanipulation of Preimplantation Embryos in Patients with Implantation Failure

Cosmetic micromanipulation is defined as fragment and coarse granulation removal from preimplantation embryos. We report two cases of pregnancies in patients with implantation failure following cosmetic micromanipulation.